Create two primers that create restrictions sites for removal and amplification of (N-Histag-T7tag-CoVprotease-C A map of the plasmid will be provided, along with the DNA sequence of the protein, which will not have a stop or start codon within it since SARS-CoV-2 creates this protein as part of a poly protein that is cleaved up later.

Using a list of restriction enzymes in the instructions Create two primers that create restrictions sites for removal and amplification of (N-Histag-T7tag-CoVprotease-C A map of the plasmid will be provided, along with the DNA sequence of the protein, which will not have a stop or start codon within it since SARS-CoV-2 creates this protein as part of a poly protein that is cleaved up later. Also, its quite difficult to find the DNA sequence on NCBI because of the lack of stop and start codons and also the researchers in the paper I will link used there own primers that are not part of the canonical sequence for the protease gene.